introduction: the aim of this study was to setup, optimize and introduce a sensitive and specific pcr detection method for identification of neisseria meningitidis dna in clinical samples. material and methods: capsular transport gene a (ctra) was selected as a specific target sequence. this primer pair amplifies 101bp of the target gene. neisseria meningitidis strain: atcc; 13090 and neisseria...

introduction: neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in human. pora is a major component of the outer membrane of n. meningitidis and functions as a cationic porin. this study aimed to clone and determine the expression of pora as the first step for producing a proper antigen in a vaccine study against n. meningitidis. methods: an approximately ...

Neisseria meningitidis is a facultative pathogen bacterium which is well founded with a number of adhesion molecules to facilitate its colonization in human nasopharynx track. Neisseria meningitidis is a major cause of mortality from sever meningococcal disease and septicemia. The Neisseria meningitidis adhesion, NadA, is a trimeric autotransporter adhesion molecule which is involved in cell ad...

background and objectives: neisseria meningitidis is transmitted from person-to-person. thus, close contact with a healthy carrier can facilitate the spread of the bacteria and lead to life-threatening meningococcal disease. the aim of this study was to identify oropharyngeal carriers of n. meningitidis in volunteers preparing for military service before vaccination. materials and methods: in a...

Computational analysis, design and expression of hybrid antibody in Single Chain Fragment Variable (scFv) form for identifying surface antigen factor H binding protein (fHbp) Neisseria meningitidis

Neisseria meningitidis serogroups A, B, C, W, X, and Y cause outbreaks of meningococcal disease. Quadrivalent conjugate vaccines targeting the are available. A pentavalent vaccine that also includes serogroup X (NmCV-5) is under development.

The adhC gene from 11 strains of Neisseria gonorrhoeae was distinguished from its homologue in Neisseria meningitidis by the presence of a premature stop codon caused by a single base insertion. Mutational analysis showed that NADH S-nitrosoglutathione oxidoreductase activity was associated with adhC in Neisseria meningitidis but not in Neisseria gonorrhoeae.

Meningococcal disease occurs as sporadic cases in developed countries, with the occasional emergence of new clones of Neisseria meningitidis. Here, we report the genome sequence of N. meningitidis strain LNP27256, an isolate of sequence type 11 linked to a recent outbreak among men who have sex with men in Europe.

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...